DAPI weirdness with hardset Vectasheild

Posted by simon walker (BI)-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DAPI-weirdness-with-hardset-Vectasheild-tp6869170.html

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Has anyone else observed weird behaviour of DAPI when used in conjunction
with hard-setting Vectasheild?  When exposed to wide-band UV excitation we
have seen the DAPI excitation characteristics change such that the DAPI
becomes excitable with 488 nm light.  And today someone showed me a slide
where (again using wide-band UV excitation) the DAPI signal initially looked a
pinky/red colour, but after a few seconds of exposure developed the usual
blue fluorescence.  We don't seem to have these problems with the liquid
mount Vectasheild.  Any suggestions as to what might be going on??
Thanks,
Simon