http://confocal-microscopy-list.275.s1.nabble.com/DAPI-weirdness-with-hardset-Vectasheild-tp6869170p6869329.html
Can you check the spectral properties using a spectrometer under the different conditions?
> Date: Fri, 7 Oct 2011 05:51:54 -0500
> From:
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> Subject: DAPI weirdness with hardset Vectasheild
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> Has anyone else observed weird behaviour of DAPI when used in conjunction
> with hard-setting Vectasheild? When exposed to wide-band UV excitation we
> have seen the DAPI excitation characteristics change such that the DAPI
> becomes excitable with 488 nm light. And today someone showed me a slide
> where (again using wide-band UV excitation) the DAPI signal initially looked a
> pinky/red colour, but after a few seconds of exposure developed the usual
> blue fluorescence. We don't seem to have these problems with the liquid
> mount Vectasheild. Any suggestions as to what might be going on??
> Thanks,
> Simon