Pixel size for confocal microscopy
Posted by bluebug on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pixel-size-for-confocal-microscopy-tp6869929.html
I am confused as to how to get the pixel size for processing a z stack from a SP5 Leica CLSM for use in another program.
Confocal experiment data:
Magn: 63 x
Dimensions = x 1024
y 1024
z 33
Physical Length Physical Origin
246.03µm 0.00 µm
246.03µm 0.00 µm
-15.81 µm 1154.90 µm