Posted by
Lloyd Donaldson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pixel-size-for-confocal-microscopy-tp6869929p6874961.html
You need to look further down the properties page to voxel width, height, and depth, not the image size values you are looking at. Alternatively you can divide the physical width by the number of pixels etc.
Dr Lloyd Donaldson
Senior Scientist, Project Leader - Microscopy/Wood Identification
Scion - Forests . Products . Innovation
Private Bag 3020, Rotorua
49 Sala Street, Rotorua 3010
New Zealand
Ph: 64 7 343 5581
www.scionresearch.com
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From: Confocal Microscopy List [mailto:
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Sent: Saturday, 8 October 2011 5:23 a.m.
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Subject: Pixel size for confocal microscopy
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I am confused as to how to get the pixel size for processing a z stack from a
SP5 Leica CLSM for use in another program.
Confocal experiment data:
Magn: 63 x
Dimensions = x 1024
y 1024
z 33
Physical Length Physical
Origin
246.03µm 0.00 µm
246.03µm 0.00 µm
-15.81 µm 1154.90 µm
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