Re: image multiwell plate on confocal microscope

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/image-multiwell-plate-on-confocal-microscope-tp6885481p6885583.html

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Dear Yue,
Have you contacted the stage manufacturer?   Most motorized stages can accept plates but you may need to buy an adapter from the manufacturer.  Be aware that the distance between the bottom of the plate and the plate's plastic skirt is a few millimeters and varies between plate brands, and styles within a brand. The skirt requires that you bring the object lens so far up to reach the bottom of the plate that the stage can strike either the imaging lens or an adjacent lens, especially when someone else gets on the system with a slide or dish.  If the stage maker does not offer a plate holder that will lower the plate's position to reduce the problem, but you can usually buy a solid blank insert which can be taken to your local machine shop.  

regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]








On Oct 12, 2011, at 8:40 AM, [hidden email] wrote:

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>
> Hello everyone,
>
> We have a Leica SP5 X confocal microscope. We are planning to image 96-well plates on it. But so far I haven't found anything to fit the plate on the stage.
>
> I'm wondering anyone on the list has experiences with this kind of experiment. Any suggestion is welcome.
>
> Thank you!
>
> Yue