Re: image multiwell plate on confocal microscope

Posted by yue_li on
URL: http://confocal-microscopy-list.275.s1.nabble.com/image-multiwell-plate-on-confocal-microscope-tp6885481p6885741.html

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Hi Glen,

I have contacted Leica, and the rep said he needed to ask around.

Thank you for the warning. I'll definitely be very careful when I  
switch stages.

best,
Yue


Quoting Glen MacDonald <[hidden email]>:

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> Dear Yue,
> Have you contacted the stage manufacturer?   Most motorized stages  
> can accept plates but you may need to buy an adapter from the  
> manufacturer.  Be aware that the distance between the bottom of the  
> plate and the plate's plastic skirt is a few millimeters and varies  
> between plate brands, and styles within a brand. The skirt requires  
> that you bring the object lens so far up to reach the bottom of the  
> plate that the stage can strike either the imaging lens or an  
> adjacent lens, especially when someone else gets on the system with  
> a slide or dish.  If the stage maker does not offer a plate holder  
> that will lower the plate's position to reduce the problem, but you  
> can usually buy a solid blank insert which can be taken to your  
> local machine shop.
>
> regards,
> Glen
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> (206) 616-4156
> [hidden email]
>
>
>
>
>
>
>
>
> On Oct 12, 2011, at 8:40 AM, [hidden email] wrote:
>
>> *****
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>>
>> Hello everyone,
>>
>> We have a Leica SP5 X confocal microscope. We are planning to image  
>>  96-well plates on it. But so far I haven't found anything to fit  
>> the plate on the stage.
>>
>> I'm wondering anyone on the list has experiences with this kind of  
>> experiment. Any suggestion is welcome.
>>
>> Thank you!
>>
>> Yue
>