Posted by
Emmanuel Gustin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/image-multiwell-plate-on-confocal-microscope-tp6885481p6886325.html
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Hello,
- Use plates specifically designed for high-content imaging. Typically these have a low skirt,
to avoid a conflict with the objective at the sides of the plate, and a decent optical quality
bottom of the correct thickness.
- On our Leica SP2, we can insert a plate holder in the (Marzhauser) stage but the scanning
range of the stage is not large enough to be able to access the entire plate. I am not sure
whether that is a physical limit or a software limit. It is worth checking this before you
purchase a plate adapter. (We did not, but we almost never use the plate adapter anyway.)
- As far as I know, plate adapters are not compatible with Leica's on-stage Z-scan unit.
Best Regards,
Emmanuel
-----Original Message-----
From: Confocal Microscopy List on behalf of
[hidden email]
Sent: Wed 2011-10-12 6:36 PM
To:
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Subject: Re: image multiwell plate on confocal microscope
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Hi Glen,
I have contacted Leica, and the rep said he needed to ask around.
Thank you for the warning. I'll definitely be very careful when I
switch stages.
best,
Yue
Quoting Glen MacDonald <
[hidden email]>:
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> Dear Yue,
> Have you contacted the stage manufacturer? Most motorized stages
> can accept plates but you may need to buy an adapter from the
> manufacturer. Be aware that the distance between the bottom of the
> plate and the plate's plastic skirt is a few millimeters and varies
> between plate brands, and styles within a brand. The skirt requires
> that you bring the object lens so far up to reach the bottom of the
> plate that the stage can strike either the imaging lens or an
> adjacent lens, especially when someone else gets on the system with
> a slide or dish. If the stage maker does not offer a plate holder
> that will lower the plate's position to reduce the problem, but you
> can usually buy a solid blank insert which can be taken to your
> local machine shop.
>
> regards,
> Glen
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923 USA
> (206) 616-4156
>
[hidden email]
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>
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>
>
> On Oct 12, 2011, at 8:40 AM,
[hidden email] wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Hello everyone,
>>
>> We have a Leica SP5 X confocal microscope. We are planning to image
>> 96-well plates on it. But so far I haven't found anything to fit
>> the plate on the stage.
>>
>> I'm wondering anyone on the list has experiences with this kind of
>> experiment. Any suggestion is welcome.
>>
>> Thank you!
>>
>> Yue
>