Posted by
lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Searching-for-a-new-hardening-mounting-medium-for-cultured-cells-tp6925980.html
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Hi,
I was a happy user of Fluorsave for mounting immunlabeled cells cultured on
coverlips. Recently though, our Fluorsave has stopped hardening after
overnight curing at RT (it has happened with our regular Fluorsave and with
another bottle from a different lot we ordered after this problem
appeared).
First, is there any user of Fluorsave that has had this problem and managed
to find an explanation/solution to it?
Second, can you recommend a good alternative for a mounting cultured cells
labelled with Alexa/DyLight fluorophores ?
I'd prefer something that does not require sealing. I had a look at Tony
Collin's "Mountants" document (
http://www.lajollaneuroscience.org/sr/homepage/cell/Mountants.pdf) and tried
ProLong Gold, which is very nice but too expensive for routine work
(something like 20 times the price of Fluorsave). I got some FluoroGel (
http://www.emsdiasum.com/microscopy/technical/datasheet/17985.aspx) and I'm
currently evaluating it.
Any other advice or mountant proposition ?
Thanks,
--
Christophe Leterrier
Postdoc
INSERM UMR641 Neurobiologie des Canaux Ioniques
IFR Jean Roche, Université de la Méditerranée
Marseille, France
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