Re: Problems with Red Safe

Posted by Claudia Campos-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Problems-with-Red-Safe-tp6927958p6931850.html

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Thanks a lot for your suggestion Cathy, maybe we should move to that reagent.

In the lab we dilute red safe in the gel (usually 30 to 60 ml volume )
according to the manufacturer instructions, and we are aware that as the
fragment gets smaller, its intensity also decreases. But I was checking for
2 fragments with 2kb and 9 kb...this should be strong enough to see...

Cheers to all

--
Cláudia Campos
Lab manager - Cell Biophysics and Development Lab
Instituto Gulbenkian Ciência
R. Quinta Grande, nº6
2781-154 Oeiras PT
Tel:+ 351 214 464 608
http://www.igc.gulbenkian.pt/research/unit/38