Posted by
James Mansfield on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Out-of-Office-autoreply-courtesy-tp6917499p6932429.html
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Hi,
There are several good online descriptions of airy disks and image resolution. Two good ones are:
http://www.microscopyu.com/tutorials/java/imageformation/airyna/http://www.olympusmicro.com/primer/java/microscopy/airydiscs2/Jim
James R. Mansfield
Director, Tissue Analysis Applications
Caliper Life Sciences, Inc.
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Need to send me large files? Use my YouSendIt DropBox:
https://dropbox.yousendit.com/Mansfield-Dropbox-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Guy Cox
Sent: Wednesday, October 26, 2011 5:46 AM
To:
[hidden email]
Subject: Airy Units - was: RE: "Out of Office autoreply" courtesy
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You are right, it has been totally drowned out!
The Airy unit is defined by the size of the Airy disk, that is the size
of the disk representing the image of a point object. Airy was an
astronomer and thus derived it by reference to stars (which, though
huge, are so far away that they appear as point objects). John Strutt,
Lord Rayleigh, proposed a general resolution criterion that two objects
can be considered resolved if the maximum of one Airy disk lies on the
first minimum of the other. This criterion, the radius of the central
disk (ignoring surrounding haloes) is given by r = 0.61 lambda /
NA, where lambda is the wavelength of the light being used.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
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http://www.guycox.net-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of Peter G. Werner
Sent: Sunday, 23 October 2011 4:55 AM
To:
[hidden email]
Subject: Re: "Out of Office autoreply" courtesy
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And I hate to point this out, but the question I originally asked
(concerning the definition of Airy Units) has been drowned out by all
the
commentary about the "Out of Office autoreply" that my initial email
generated.
If nobody has an answer to the question, no worries, but I'd hate to see
the
topic get lost under the weight of discussion of listserv function.
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