Deconvolution of Confocal Images? (was: Airy Units)

Posted by Peter Werner on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-Confocal-Images-was-Airy-Units-tp6946310.html

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An interesting point was made here by Jim Pawley:

> I agree that sampling a bit higher than Nyquist never hurts,  
> especially if you deconvolve (as you always should), but I think  
> that it is a mistake to think that one can "separate" out the noise  
> by decon. I think that noise is pretty fundamental.

I had always heard that if you're doing confocal microscopy, at least  
point-scanning confocal with a pinhole size of 1AU or smaller, that  
deconvolution was superfluous, because you shouldn't be getting out of  
focus light. So what is gained by deconvolution when one is sampling  
voxel by voxel?

Peter G. Werner
Merritt College Microscopy Program