Re: Rejected posting to [e-mail]

Posted by Axel Kurt Preuss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064p6957836.html

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Dear Hanna
Koehler comes to mind as already suggested.
I m not familiar with scanning station

We had similar problem on an Olympus scope and found that the glass in front of the   transmission lamp had a crack (heat?)

So, this  glass  in the transmission housing, which directly faces the microscope stage , had a crack and that produced a shadow in the image pretending to be a Koehler problem

cheers

Axel                    6-19B ,   92715622



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hanna Sas Nowosielska
Sent: Thursday, 6 October, 2011 10:37 PM
To: [hidden email]
Subject: Re: Rejected posting to [hidden email]

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Yes, at least Olymus  claims everything with the optical path is ok.

Hanna

2011/10/6 tineke vendrig <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Have you optimised you microscope ?? Kohler illumination and so on..??
>
> Tineke Vendrig, ing
> technical engeneer optical microscopy
> Delft University of Technology
> Bionano Science
> Kavli Institute of Nanoscience
> Lorentzweg 1
> 2628LJ Delft
> room F185
> Tel: [phone]
> Fax:[phone]
> email: [hidden email]
> mobile phone: [phone]
>
>
> 2011/10/6 Hanna Sas Nowosielska <[hidden email]>
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear all,
>>
>> For a couple of months we are struggling with a serious problem with
>> the field illumination in our Olympus Scan^R Scanning station. The
>> image of the specimen, when observed on the computer screen is much
>> darker on one side than on the other. We thought it is the lamp but
>> the lamp is new at properly inserted. Then we tried calibrating the
>> optic fiber, but it didn't help at all. Our third guess was the
>> filters.We checked all of the filters  -  the coating has lots of
>> small dark spots with biggest concentration of the spots in the
>> middle of the filters, but I am concerned if that can  produce uneven
>> illumination of the field so that only one part (let say left corner)
>> is underilluminated? So, I turn to you and your experience: did may
>> anyone have the similar problem? Do you have any suggestion what can
>> be the cause of the problem?
>>
>> Best regards,
>> Hanna
>>
>> --
>> Department of Plant Anatomy and Cytology Faculty of Biology and
>> Environmental Protection University of Silesia Jagiellonska Str. 28
>> 40-032 Katowice
>> Poland
>>
>
>
>
> --
> Tineke Vendrig, ing
> technical engeneer optical microscopy
> Delft University of Technology
> Bionano Science
> Kavli Institute of Nanoscience
> Lorentzweg 1
> 2628LJ Delft
> room F185
> Tel: [phone]
> Fax:[phone]
> email: [hidden email]
> mobile phone: [phone]
>

--
Department of Plant Anatomy and Cytology Faculty of Biology and Environmental Protection University of Silesia Jagiellonska Str. 28
40-032 Katowice
Poland

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