Re: Rejected posting to [e-mail]

Posted by Zac Arrac Atelaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064p6964406.html

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I was thinking on this one, the uneveness of the field is more visible in a specific wavelength or in all of them? usually UV is the most uneven. A different case might be the DIC ilumination being in the wrong position (usually it has 2 positions), the other one would be a crack in the fiber (hopefully this one is just a wild guess) Best luck Gabriel OH
 > Date: Thu, 3 Nov 2011 11:46:58 +0800

> From: [hidden email]
> Subject: Re: Rejected posting to [hidden email]
> To: [hidden email]
>
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> Dear Hanna
> Koehler comes to mind as already suggested.
> I m not familiar with scanning station
>
> We had similar problem on an Olympus scope and found that the glass in front of the   transmission lamp had a crack (heat?)
>
> So, this  glass  in the transmission housing, which directly faces the microscope stage , had a crack and that produced a shadow in the image pretending to be a Koehler problem
>
> cheers
>
> Axel                    6-19B ,   92715622
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hanna Sas Nowosielska
> Sent: Thursday, 6 October, 2011 10:37 PM
> To: [hidden email]
> Subject: Re: Rejected posting to [hidden email]
>
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> Yes, at least Olymus  claims everything with the optical path is ok.
>
> Hanna
>
> 2011/10/6 tineke vendrig <[hidden email]>:
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> > Have you optimised you microscope ?? Kohler illumination and so on..??
> >
> > Tineke Vendrig, ing
> > technical engeneer optical microscopy
> > Delft University of Technology
> > Bionano Science
> > Kavli Institute of Nanoscience
> > Lorentzweg 1
> > 2628LJ Delft
> > room F185
> > Tel: [phone]
> > Fax:[phone]
> > email: [hidden email]
> > mobile phone: [phone]
> >
> >
> > 2011/10/6 Hanna Sas Nowosielska <[hidden email]>
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Dear all,
> >>
> >> For a couple of months we are struggling with a serious problem with
> >> the field illumination in our Olympus Scan^R Scanning station. The
> >> image of the specimen, when observed on the computer screen is much
> >> darker on one side than on the other. We thought it is the lamp but
> >> the lamp is new at properly inserted. Then we tried calibrating the
> >> optic fiber, but it didn't help at all. Our third guess was the
> >> filters.We checked all of the filters  -  the coating has lots of
> >> small dark spots with biggest concentration of the spots in the
> >> middle of the filters, but I am concerned if that can  produce uneven
> >> illumination of the field so that only one part (let say left corner)
> >> is underilluminated? So, I turn to you and your experience: did may
> >> anyone have the similar problem? Do you have any suggestion what can
> >> be the cause of the problem?
> >>
> >> Best regards,
> >> Hanna
> >>
> >> --
> >> Department of Plant Anatomy and Cytology Faculty of Biology and
> >> Environmental Protection University of Silesia Jagiellonska Str. 28
> >> 40-032 Katowice
> >> Poland
> >>
> >
> >
> >
> > --
> > Tineke Vendrig, ing
> > technical engeneer optical microscopy
> > Delft University of Technology
> > Bionano Science
> > Kavli Institute of Nanoscience
> > Lorentzweg 1
> > 2628LJ Delft
> > room F185
> > Tel: [phone]
> > Fax:[phone]
> > email: [hidden email]
> > mobile phone: [phone]
> >
>
>
>
> --
> Department of Plant Anatomy and Cytology Faculty of Biology and Environmental Protection University of Silesia Jagiellonska Str. 28
> 40-032 Katowice
> Poland
>
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