FRET-FLIM question

Posted by Straatman, Kees (Dr.) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Researcher-postdoc-National-Imaging-Platform-Oslo-tp7000581p7003613.html

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Dear list members,

We have a discussion about FRET-FLIM at the moment and somebody has published that they see an increase in FRET efficiency and argues that this is because there is more dimerisation between the two proteins they are interested in. Now, I thought that FRET-FLIM is independent from the concentration of the fluorochromes and that the increase in FRET efficiency is caused by a change in distance between the 2 reporter FP (CFP and YFP) and it would be possible to have less dimerisation of the proteins but an increase in FRET efficiency. Is this correct?

Thanks

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
College of Medicine, Biological Sciences and Psychology
University of Leicester

http://www.le.ac.uk/biochem/microscopy/home.html