Re: DIC or Phase contrast for image morphometry mesasurements?

Posted by Theresa Swayne on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-or-Phase-contrast-for-image-morphometry-mesasurements-tp7068286p7068342.html

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Hi Monique,

If fluorescence is a possibility, the work of the Ohya and Morishita labs (using fluorescent ConA to mark the cell wall) might be helpful. They also wrote a program called CalMorph to analyze many cell size and shape parameters.

Good luck,
Theresa

On Dec 6, 2011, at 3:23 PM, Vasseur Monique wrote:

>
> I wonder what is better for yeast morphometry shape and size image analysis:
> should we better use phase contrast images or DIC images?

------------------------------------
Theresa C. Swayne, Ph.D.
Manager, Confocal and Specialized Microscopy Shared Resource
Herbert Irving Comprehensive Cancer Center, Columbia University
1130 Saint Nicholas Ave, 222A
New York, NY 10032

212-851-4613

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