Re: DIC or Phase contrast for image morphometry mesasurements?

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-or-Phase-contrast-for-image-morphometry-mesasurements-tp7068286p7068353.html

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Hi Monique, what exactly would you like to measure? We have a method for volume measurements, if this would be of interest. Some of it has been published, but I can send you the latest information. - Mike

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vasseur Monique
Sent: Tuesday, December 06, 2011 3:23 PM
To: [hidden email]
Subject: DIC or Phase contrast for image morphometry mesasurements?

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Dear All,

I wonder what is better for yeast morphometry shape and size image analysis:
should we better use phase contrast images or DIC images? Is one more
accurate  for measures? )Which threshold will be more precise: the one of DIC
shear or the one of phase contrast halo?)  Do some of you have experience on
this question? Any input is welcome  Thanks a lot!
Monique