Posted by
G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-or-Phase-contrast-for-image-morphometry-mesasurements-tp7068286p7068719.html
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I did a lot of yeast imaging with DIC in grad. school and it was a
pain for morphometry because of the "shadow" effect of DIC; one side
of the cells was bright while the other was dark and that was
problematic for thresholding. Now I'd use phase, or even brightfield
or darkfield. From your question, though, it sounds like getting the
cell outline is no problem for you with DIC and you're just wondering
about accuracy, which I don't know.
-Esteban
On Tue, Dec 6, 2011 at 12:23 PM, Vasseur Monique
<
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> Dear All,
>
> I wonder what is better for yeast morphometry shape and size image analysis:
> should we better use phase contrast images or DIC images? Is one more
> accurate for measures? )Which threshold will be more precise: the one of DIC
> shear or the one of phase contrast halo?) Do some of you have experience on
> this question? Any input is welcome Thanks a lot!
> Monique