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Monique Vasseur on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-or-Phase-contrast-for-image-morphometry-mesasurements-tp7068286p7070620.html
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Hi Mike,
We are trying to caracterize and classify different yeast mutants by their morphology appearance (morphotype) and size. Actually, we are trying to caracterize in 2D with the different shape factors like circularity, elongation, aspect ratio, sphericity... and so on, and by the area. For your volume measurements do you need 3D acquisition? I guess volume should be more precise than area. Yes, your publication interests me. Thanks a lot
All the best,
Monique Vasseur
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De : Confocal Microscopy List [mailto:
[hidden email]] De la part de MODEL, MICHAEL
Envoyé : 6 décembre 2011 15:53
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Objet : Re: DIC or Phase contrast for image morphometry mesasurements?
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Hi Monique, what exactly would you like to measure? We have a method for volume measurements, if this would be of interest. Some of it has been published, but I can send you the latest information. - Mike
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Sent: Tuesday, December 06, 2011 3:23 PM
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Subject: DIC or Phase contrast for image morphometry mesasurements?
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Dear All,
I wonder what is better for yeast morphometry shape and size image analysis:
should we better use phase contrast images or DIC images? Is one more
accurate for measures? )Which threshold will be more precise: the one of DIC
shear or the one of phase contrast halo?) Do some of you have experience on
this question? Any input is welcome Thanks a lot!
Monique