Re: DIC or Phase contrast for image morphometry mesasurements?

Posted by Peter Werner on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-or-Phase-contrast-for-image-morphometry-mesasurements-tp7068286p7073422.html

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Vasseur Monique wrote:

> I wonder what is better for yeast morphometry shape and size image  
> analysis:
> should we better use phase contrast images or DIC images? Is one more
> accurate  for measures? )Which threshold will be more precise: the  
> one of DIC
> shear or the one of phase contrast halo?)  Do some of you have  
> experience on
> this question? Any input is welcome  Thanks a lot!
> Monique

I'd say whatever technique you use, avoid phase contrast for  
morphometry due to haloing. In my experience, it's often practically  
impossible to define an "edge" in a phase contrast image. As stated  
elsewhere, simple bright-field can provide very accurate morphometry  
if the cells have sufficient innate contrast, or if they can be  
stained, though I'm not sure if your protocol allows for that.

Peter G. Werner
Merritt College Microscopy Program