Re: small and variable working distance

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/last-seats-for-free-Diaspro-Lab-School-on-Super-resolution-in-Genoa-Istituto-Italiano-di-Tecnologia-tp7073663p7076707.html

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Dear Mike--

How close do you need to get and what kind of resolution do you need?
And in what medium?  Air?  Water?  Oil?

Good luck!

Martin

On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:

> Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
>> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might
> help me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf

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