Re: small and variable working distance

Posted by Guy Cox-2 on
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These are known as solid immersion lenses, and they certainly do exist.  From the little that I understand the idea is that the lens is within the evanescent field so that the refractive index appears to be continuous to the sample.  I'm sure there optics people on this list who could give a better explanation.   Whether you could get one to fit a standard microscope is a different question entirely, but if you are working on an optical bench I'd imagine the usual suspects could oblige.  

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Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL
Sent: Friday, 9 December 2011 8:47 AM
To: [hidden email]
Subject: Re: small and variable working distance

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Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Thursday, December 08, 2011 4:27 PM
To: [hidden email]
Subject: Re: small and variable working distance

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Dear Mike--

On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:

> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!

I'm not sure I'm getting the concept--do you want an objective that
*needs* to be in contact with the specimen to be in focus?  --It might
help me if you explained a bit more about what you're trying to do.

Thanks--

Martin Wessendorf
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