Re: small and variable working distance

Posted by Arne Seitz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/last-seats-for-free-Diaspro-Lab-School-on-Super-resolution-in-Genoa-Istituto-Italiano-di-Tecnologia-tp7073663p7077170.html

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Hi Mike,
if you want to bring the objective as close to the object as possible maybe Scanning Near-field Optical microscopy (SNOM) could do the job.

Cheers Arne

---------------------------------------------------------------
Dr. Arne Seitz
Head of Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 15, AI 0241
CH-1015 Lausanne

Phone: +41 21 693 9618
Fax:      +41 21 693 9585
http://biop.epfl.ch/
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> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of MODEL,
> MICHAEL
> Sent: jeudi 8 décembre 2011 22:47
> To: [hidden email]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Martin - that's correct, I want to bring the objective very close to the
> specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Martin
> Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
>
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
> > Dear Confocal Microscopists - Does anyone of an objective with variable,
> down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might help
> me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: (612) 626-0145
> Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
> University of Minnesota             Preferred FAX: (612) 624-8118
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