Re: small and variable working distance

Posted by Jeremy Adler-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/last-seats-for-free-Diaspro-Lab-School-on-Super-resolution-in-Genoa-Istituto-Italiano-di-Tecnologia-tp7073663p7078045.html

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Out of interest, is the limitation that the water layer is only 20-30  
microns thick or that the objective really needs to be that close ?

a 20-30 micron thick layer of water can easily by sandwiched between a  
slide and a coverslip using microspheres as spaces. Then image with a  
water objective that works thru a coverslip.




Quoting "MODEL, MICHAEL" <[hidden email]>:

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> 20-30 microns in water. I doubt such lenses exist but who knows... Thanks!
>
> Mike
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On  
> Behalf Of Martin Wessendorf [[hidden email]]
> Sent: Thursday, December 08, 2011 9:20 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
>
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> Dear Mike--
>
> How close do you need to get and what kind of resolution do you need?
> And in what medium?  Air?  Water?  Oil?
>
> Good luck!
>
> Martin
>
> On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:
>
>> Hi Martin - that's correct, I want to bring the objective very  
>> close to the specimen, almost touching it.
>>
>> -----Original Message-----
>> From: Confocal Microscopy List  
>> [mailto:[hidden email]] On Behalf Of Martin  
>> Wessendorf
>> Sent: Thursday, December 08, 2011 4:27 PM
>> To: [hidden email]
>> Subject: Re: small and variable working distance
>> Dear Mike--
>>
>> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>>
>>> Dear Confocal Microscopists - Does anyone of an objective with  
>>> variable, down to zero, working distance? Thanks!
>>
>> I'm not sure I'm getting the concept--do you want an objective that
>> *needs* to be in contact with the specimen to be in focus?  --It might
>> help me if you explained a bit more about what you're trying to do.
>>
>> Thanks--
>>
>> Martin Wessendorf
>
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Jeremy Adler
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