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Guy Cox-2 on
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The only way this would be possible is if it were stopped down to reduce its actual aperture in glycerine. This is technically possible but (IMHO) unlikely. Glycerol has a refractive index of 1.4722 at 590nm (the closest I can find to the official 550nm). NA 1.3 therefore corresponds to an acceptance half-angle of 62 degrees. Water at 550nm has a refractive index of 1.33. This same half-angle would be an NA of 1.17.
Looking at it the other way round, to achieve NA 1.3 in water requires an acceptance angle of 77 degrees, which while not impossible is pushing the envelope.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Xiao Liu
Sent: Friday, 16 December 2011 11:09 AM
To:
[hidden email]
Subject: resolution of Zeiss Water and Glycerine 63X lens
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Hi, everyone:
I'm looking for a 63X objective for live imaging and its resolution is my major concern. Zeiss recommands Objective LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27, saying it has NA = 1.3 in both water and glycerine. Does it really have NA=1.3 even in water?
Your reply is highly appreciated.
Xiao
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Xiao Liu
School of Life Sciences
Tsing-Hua University
Beijing, China
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