Re: chromatic aberration

Posted by mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Concentrating-bacteria-cells-for-microscope-visualization-tp6519025p7134789.html

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Every objective needs to be tested with beads with the wavelengths you are using.  Any time we need precise XY or Z axis registration we test with beads or fluorescent coatings on the surfaces in the same media as the biological imaging, sometimes even on the cells.  Years ago we found out the hard way, when doing CFP/YFP ratio FRET, that there is no substitute for characterizing each objective as close to the real imaging conditions as practical.  Generalizations such as, "High NA brand X planapo objective are fine," just are not true.
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Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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