Re: Light Lab by Carl Zeiss Microscopy for iOS devices

Posted by Cameron Nowell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-Lab-by-Carl-Zeiss-Microscopy-for-iOS-devices-tp7184469p7194238.html

One other thing, the calculator asks for the back projected pinhole size. You can calculate that here http://www.svi.nl/BackprojectedPinholeCalculator


Cheers

Cam



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steffen Dietzel
Sent: Tuesday, 17 January 2012 12:06 AM
To: [hidden email]
Subject: Re: Light Lab by Carl Zeiss Microscopy for iOS devices

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Well, for my part I wouldn't mind an (Android) app for

- z-resolution, prefereably one that includes 2- and 3-photon excitation and

- resolution and brightness at various depths under refraction index mismatch conditions (e.g. sample embedded in glycerol instead of immersion oil or underneath a coverslip with a dipping objective).

Life isn't always as simple as 0.61 lambda/NA.


Even better though than a bunch of apps for various systems would a calculator running in a web browser based on common standards so that it would be accessible on every system.


Steffen


On 16.01.2012 02:08, Guy Cox wrote:

> There isn't any difference between widefield and confocal resolution in any normal fluorescence mode.   Do you really need an app to work out 0.61 lambda/NA?  And then to divide by 2.3 to get Nyquist?  If you are in multiphoton it's a little more complicated since root 2 (1.414) comes in, but you can just use the formula 0.43 lambda / NA.
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor&  Francis
>       http://www.guycox.com/optical.htm 
> ______________________________________________
> Guy Cox, MA, DPhil(Oxon), Honorary Associate, Australian Centre for
> Microscopy&  Microanalysis, Madsen Building F09, University of Sydney,
> NSW 2006
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