Re: 96 well - glass bottom dishes - glass unadheres

Posted by Sylvie Le Guyader-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/96-well-glass-bottom-dishes-glass-unadheres-tp720452p721318.html

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Hi Dan

You could try to fix your cells, remove the wells and the leftover glue then
circle your cultures with a PapPen (Hydrophobic) then do your staining. It
works very well and you would need far less reagents than with the wells.

Med vänlig hälsning / Best regards
 
Sylvie
 
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Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
[phone]

> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Dan Tom
> Sent: 12 August 2008 22:31
> To: [hidden email]
> Subject: 96 well - glass bottom dishes - glass unadheres
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi List,
>
> A user from my facility is having a difficulties staining cells on 96 well
> glass bottom plates.  The glue that adheres the glass to the dish

dissolves
> during the staining process causing the media to leak out.
>
> The user has fixed the cells with and without methanol, which the
> manufacturer indicates as having good compatibility, and they also tried
> paraformaldehyde.  Each time, the glue dissolves.  The current working
> hypothesis is that the refrigeration process might affected the adhesion.
>
> Could someone with a similar experience suggest what we can be doing
wrong?
>  I'm sorry I don't have any more details.  Any suggestions would be
appreciated.

>
> Thanks,
> Dan
>
> =========================
>
> Daniel Tom
>
> Harvard NeuroDiscovery Center
>
> Optical Imaging Facility
>
> 220 Longwood Ave.
>
> Goldenson Bldg., rm. 501
>
> t: 617-432-1442