Re: localization precision in PALM/STORM

Posted by Jean-Yves Tinevez-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/localization-precision-in-PALM-STORM-tp7219699p7220912.html

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On 24-Jan-12 15:43, Christian Soeller wrote:

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> You basically want to convert the AD counts in your image into photon numbers. That requires several bits of info about the camera. We have Andor cameras and I can look up the required values from the data/test sheet that comes with the individual camera (these change from cam to cam). You need to know the
> - electrons per count
> - absolute EM gain (with the quantEM you might have to measure/calibrate this)
> - A/D offset (this can be measured in dark frames with no light impinging on the cam, i.e. shutter closed)
>

Dear all,

Christophe wants to estimate the localization precision dependance on
the detected photons, so only the photo-electrons matter. Therefore, you
can simply deduce what they are for each pixel using simple pixel
fluctuation, or variance estimates. You do not need the quantum efficiency.

Best
jyt


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Jean-Yves Tinevez
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