Re: How "deep" can you see in a brain slice?

Posted by Hugh Newman on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-deep-can-you-see-in-a-brain-slice-tp7256661p7256737.html

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I'm no medical guy but I work for Japanese microscope company that will not be named.
Brain isn't very translucent so I wouldn't hold out much hope. I would say 100um tops, maybe even closer to 50um. After this your image will start to degrade quite a bit. Any deeper than that and you are going to want a 2P-Confocal. I've imaged colloidal suspensions that are ~100um thick, and they are very close to being index matched. At the maximum depth the image wasn't that pretty.

Maybe some more Neuro people will have a better answer for you.

Good luck

H

Hugh Newman
 
Graduate Researcher

Dept. Physics and Physical Oceanography

Memorial University

St. Johns, Newfoundland, Canada

> Date: Sun, 5 Feb 2012 13:53:33 -0600
> From: [hidden email]
> Subject: How "deep" can you see in a brain slice?
> To: [hidden email]
>
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> To join, leave or search the confocal microscopy listserv, go to:
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> Dear all,
> I am trying to view fluorescently labeled glioma cells invading into a 400um
> thick brain slice on an Olypus FV300. Has anyone experience with this and
> how "deep" it is reasonable to expect to see in the slice using a confocal
> microscope? How can you maximize this depth? (selection of objectives,
> processing of the slice....)
> Thanks for any suggestions, Petr.
>
> Petr Busek, MD, PhD
> Charles University in Prague
> First Faculty of Medicine
> Laboratory of Cancer Cell Biology
> Institute of Biochemistry and Experimental Oncology
> U Nemocnice 5
> 128 53 Prague 2
> Czech Republic
> www.lf1.cuni.cz/lbnb
>  Fax [phone]