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Re: How "deep" can you see in a brain slice?

Posted by ChrisWilms on Feb 05, 2012; 11:20pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-deep-can-you-see-in-a-brain-slice-tp7256661p7257012.html

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 From my experience using a 488 nm laser line, you can expect to get  
about 80 microns into the slice tissue.

For anything deeper, I would recommend moving to far red dyes and  
excitation and beyond that 2P.

Best of luck,

Christian

> Dear all,
> I am trying to view fluorescently labeled glioma cells invading into  
> a 400um
> thick brain slice on an Olypus FV300. Has anyone experience with  
> this and
> how "deep" it is reasonable to expect to see in the slice using a  
> confocal
> microscope? How can you maximize this depth? (selection of objectives,
> processing of the slice....)
> Thanks for any suggestions, Petr.