Re: How "deep" can you see in a brain slice?
Posted by
ChrisWilms on
Feb 05, 2012; 11:20pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-deep-can-you-see-in-a-brain-slice-tp7256661p7257012.html
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From my experience using a 488 nm laser line, you can expect to get
about 80 microns into the slice tissue.
For anything deeper, I would recommend moving to far red dyes and
excitation and beyond that 2P.
Best of luck,
Christian
> Dear all,
> I am trying to view fluorescently labeled glioma cells invading into
> a 400um
> thick brain slice on an Olypus FV300. Has anyone experience with
> this and
> how "deep" it is reasonable to expect to see in the slice using a
> confocal
> microscope? How can you maximize this depth? (selection of objectives,
> processing of the slice....)
> Thanks for any suggestions, Petr.