Re: How "deep" can you see in a brain slice?

Posted by Barbara Foster on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-deep-can-you-see-in-a-brain-slice-tp7256661p7257718.html

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Hi,

I was shocked when I visited Neuroscience this past Fall and saw what
they were doing with special clearing agents and special objectives
with multiphoton.  The new limit is.... (ready?) at least 4 mm (no,
that is not microns) and I think that there may be some new
combinations which go as far as 8mm. This will be one of the topics
for the Editor's Page for the April issue of American Lab.  I will be
writing that article this next week and can get you the current stats then.

Good hunting!
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
P: [phone]
W: www.MicroscopyEducation.com

We are now scheduling courses through June 2012

At 12:08 PM 2/5/2012, you wrote:

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>
>I'm no medical guy but I work for Japanese microscope company that
>will not be named.
>Brain isn't very translucent so I wouldn't hold out much hope. I
>would say 100um tops, maybe even closer to 50um. After this your
>image will start to degrade quite a bit. Any deeper than that and
>you are going to want a 2P-Confocal. I've imaged colloidal
>suspensions that are ~100um thick, and they are very close to being
>index matched. At the maximum depth the image wasn't that pretty.
>
>Maybe some more Neuro people will have a better answer for you.
>
>Good luck
>
>H
>
>Hugh Newman
>
>Graduate Researcher
>
>Dept. Physics and Physical Oceanography
>
>Memorial University
>
>St. Johns, Newfoundland, Canada
>
>
>
> > Date: Sun, 5 Feb 2012 13:53:33 -0600
> > From: [hidden email]
> > Subject: How "deep" can you see in a brain slice?
> > To: [hidden email]
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear all,
> > I am trying to view fluorescently labeled glioma cells invading
> into a 400um
> > thick brain slice on an Olypus FV300. Has anyone experience with this and
> > how "deep" it is reasonable to expect to see in the slice using a confocal
> > microscope? How can you maximize this depth? (selection of objectives,
> > processing of the slice....)
> > Thanks for any suggestions, Petr.
> >
> > Petr Busek, MD, PhD
> > Charles University in Prague
> > First Faculty of Medicine
> > Laboratory of Cancer Cell Biology
> > Institute of Biochemistry and Experimental Oncology
> > U Nemocnice 5
> > 128 53 Prague 2
> > Czech Republic
> > www.lf1.cuni.cz/lbnb
> >  Fax [phone]
>