Re: How "deep" can you see in a brain slice?

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-deep-can-you-see-in-a-brain-slice-tp7256661p7260904.html

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Hi Barbara,

Olympus now has 2 mm, 4 mm and 8 mm working distance "Scale" objective
lenses. Pricey.

Recent Nature product focus - oppostie page 535 of the 26 January 2012
issue is a Zeiss plan-apochromatic 20x/1.0 VIS-IR lens with working
distance of 5.6 mm.

The Erturk et al paper used both a multiphoton/confocal and the
Ultramicroscope from LaVision Biotec -
http://www.lavisionbiotec.com/en/microscopy-products/ultramicroscope/   
... My next door neighbors (at work) are getting their Ultramicroscope
delivered in March. I don't know which model. Don't know when/if I'll
get to see it head to head with the Leica MP/SP5 I manage (my neighbors
have their own confocal scope so don't come over much).

Enjoy,

George




On 2/5/2012 11:12 PM, Barbara Foster wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> Hi,
>
> I was shocked when I visited Neuroscience this past Fall and saw what
> they were doing with special clearing agents and special objectives
> with multiphoton.  The new limit is.... (ready?) at least 4 mm (no,
> that is not microns) and I think that there may be some new
> combinations which go as far as 8mm. This will be one of the topics
> for the Editor's Page for the April issue of American Lab.  I will be
> writing that article this next week and can get you the current stats
> then.
>
> Good hunting!
> Barbara Foster, President and Sr. Consultant
>
> Microscopy/Microscopy Education
> P: [phone]
> W: www.MicroscopyEducation.com
>
> We are now scheduling courses through June 2012
>
> At 12:08 PM 2/5/2012, you wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>>
>> I'm no medical guy but I work for Japanese microscope company that
>> will not be named.
>> Brain isn't very translucent so I wouldn't hold out much hope. I
>> would say 100um tops, maybe even closer to 50um. After this your
>> image will start to degrade quite a bit. Any deeper than that and you
>> are going to want a 2P-Confocal. I've imaged colloidal suspensions
>> that are ~100um thick, and they are very close to being index
>> matched. At the maximum depth the image wasn't that pretty.
>>
>> Maybe some more Neuro people will have a better answer for you.
>>
>> Good luck
>>
>> H
>>
>> Hugh Newman
>>
>> Graduate Researcher
>>
>> Dept. Physics and Physical Oceanography
>>
>> Memorial University
>>
>> St. Johns, Newfoundland, Canada
>>
>>
>>
>> > Date: Sun, 5 Feb 2012 13:53:33 -0600
>> > From: [hidden email]
>> > Subject: How "deep" can you see in a brain slice?
>> > To: [hidden email]
>> >
>> > *****
>> > To join, leave or search the confocal microscopy listserv, go to:
>> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> > *****
>> >
>> > Dear all,
>> > I am trying to view fluorescently labeled glioma cells invading
>> into a 400um
>> > thick brain slice on an Olypus FV300. Has anyone experience with
>> this and
>> > how "deep" it is reasonable to expect to see in the slice using a
>> confocal
>> > microscope? How can you maximize this depth? (selection of objectives,
>> > processing of the slice....)
>> > Thanks for any suggestions, Petr.
>> >
>> > Petr Busek, MD, PhD
>> > Charles University in Prague
>> > First Faculty of Medicine
>> > Laboratory of Cancer Cell Biology
>> > Institute of Biochemistry and Experimental Oncology
>> > U Nemocnice 5
>> > 128 53 Prague 2
>> > Czech Republic
>> > www.lf1.cuni.cz/lbnb
>> >  Fax [phone]
>>
>

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami