Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/upcoming-CW-STED-demo-in-College-Station-TX-tp7268179p7269079.html
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Hi Stan,
You may want to order a STAR 440XS reagent(s) from Abberior.com - i.e.
an antibody conjugate - to evaluate as a second color (458 nm
excitation). The Pacific Orange and ATTO 425 we used during our
August-September demo in Miami performed poorly.
I hope Leica has replaced its pathetic demo vibration isolation table
with a table that is useful - our custom STED proposals in September
specified a 300 mm thick TMC table instead of Leica's demo or quote
tables (Leica confocal, B-Halle.de 3D-EasySTED segmented waveplates,
Coherent Chameleon Ultra II + OPO-Vis. estimate 50x50x210 nm XYZ ... see
the Abberior web site for their segmented waveplates and).
Alexa Fluor 488 and EGFP worked well - when the building was not
vibrating the microscope.
The leica demo machine should have at least one HyD detector - very nice.
George
p.s. if you have mouse brain users - see PubMed 22301313.
For more on 3D-EasySTED, my spreadsheet at
http://works.bepress.com/gmcnamara/3/ has the web address for
Matthias Reuss's PhD dissertation - related work in PubMed 22109398.
On 2/8/2012 4:20 PM, Stanislav Vitha wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hallo,
> for colleagues located in the East Central Texas, I would like to let you know
> that out imaging facilty will have a 2.5-week demo of Leica CW STED system,
> as well as some other instruments, starting Feb-15, 2012.
> If you are in the area and would be interested in visiting the demo, please
> contact me.
>
> More info at
http://microscopy.tamu.edu/whats-new>
> With regards,
>
> Stan Vitha
>
> Dr. Stanislav Vitha
>
[hidden email]
> Microscopy and Imaging Center
> Texas A&M University
> BSBW 119
> College Station, TX 77843-2257
>
>
http://microscopy.tamu.edu>
> tel: 979-845-1129 (main desk)
> tel: 979-845-1607 (direct)
> fax: 979-847-8933
>
>
>
--
George McNamara, PhD
Analytical Imaging Core Facility
University of Miami