Re: LEDs

Posted by jacques on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p7307846.html

Hi all,

two years later, and a quite similar question.

I use a Zeiss epifluorescence microscope, and I'm thinking to replace my HBO housing by a LED multicolor source.
Of course, Zeiss sells the colibri setup, but the choice on the market is growing day after day, and for example, Thorlabs also sells something with 4 colors and a control unit, with the Zeiss mounting system.
I'm sure many other manufacturers do the same.

Do anybody compared the HBO to a LED system for classical epifluorescence ? What should be the LED power to have something similar to a 100W at common wavelenghts (dapi, gfp, rhodamine, etc) ? Even after checking this page :
http://www.olympusfluoview.com/theory/noncoherentsources.html
It is still difficult to know which valeus and units should be considered...

Thanks for the help

J.