Re: LEDs

Posted by Gregg Jarvis on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p7308203.html

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Jacques,
Be careful what you buy.
Some LED lights have out of band leaks that cause other colors to be seen
by your detector and the watts provided are a bit different in color
correction.
This might not be true for your application, but food for thought
Gregg Jarvis
Senior Spectroscopist
Omega Optical Inc.
Brattleboro, VT. 05301
[hidden email]



On Wed, Feb 22, 2012 at 5:07 AM, jacques <[hidden email]> wrote:

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>
> Hi all,
>
> two years later, and a quite similar question.
>
> I use a Zeiss epifluorescence microscope, and I'm thinking to replace my
> HBO
> housing by a LED multicolor source.
> Of course, Zeiss sells the colibri setup, but the choice on the market is
> growing day after day, and for example, Thorlabs also sells something with
> 4
> colors and a control unit, with the Zeiss mounting system.
> I'm sure many other manufacturers do the same.
>
> Do anybody compared the HBO to a LED system for classical epifluorescence ?
> What should be the LED power to have something similar to a 100W at common
> wavelenghts (dapi, gfp, rhodamine, etc) ? Even after checking this page :
> http://www.olympusfluoview.com/theory/noncoherentsources.html
> It is still difficult to know which valeus and units should be
> considered...
>
> Thanks for the help
>
> J.
>
>
> -----
>
> Jacques FATTACCIOLI
>
> Département de Chimie
> Ecole Normale Supérieure
> 24 rue Lhomond 75231 Paris Cedex 05
> Email : [hidden email]
> Web : http://jacquesfattaccioli.wordpress.com
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