Posted by
Justin Ross on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p7313706.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi,
We previously had the Xe lamps and changed to the SSI illumination on
our Deltavision Core. The exposure times were reduced by a factor of 5
to 10. The SSI is actually made by Lumencor (no commercial interest).
From my experience it works really well without the downsides of a lamp
of heat generation or having to align and change bulbs.
If you do decide to use LEDs, I strongly suggest still using bandpass
excitation filter (as suggested previously) because with some LEDs there
upto 5% light emission at wavelengths upto 50 nm higher than the peak
which will generally add a lot of background to your images. If you have
a spectrometer available I suggest scanning the emission to get the real
emission spectrum of the LED.
Regards,
Justin.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of Julio Vazquez
Sent: Friday, 24 February 2012 4:14 AM
To:
[hidden email]
Subject: Re: LEDs
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hello all,
Applied Precision just told me that the InsightSSI illuminator is
actually not LED based, so please ignore my previous posting, as far as
LEDs are concerned.
Julio.
==
On Feb 23, 2012, at 2:06 AM, jacques wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Thanks for your help and comments !
>
> Jacques