Posted by
Boswell, Carl A - (cboswell) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p7313816.html
"If you have a spectrometer available I suggest scanning the emission to get the real emission spectrum of the LED."
It would be of more general use if the manufacturer supplied a standardized, trustworthy spectral output of these devices. Then everyone could determine for themselves what would work best for their systems and samples without the need for additional instrumentation.
C
Carl A. Boswell
520-742-6131
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Justin Ross
Sent: Thursday, February 23, 2012 6:14 PM
To:
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Subject: Re: LEDs
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Hi,
We previously had the Xe lamps and changed to the SSI illumination on our Deltavision Core. The exposure times were reduced by a factor of 5 to 10. The SSI is actually made by Lumencor (no commercial interest).
From my experience it works really well without the downsides of a lamp of heat generation or having to align and change bulbs.
If you do decide to use LEDs, I strongly suggest still using bandpass excitation filter (as suggested previously) because with some LEDs there upto 5% light emission at wavelengths upto 50 nm higher than the peak which will generally add a lot of background to your images. If you have a spectrometer available I suggest scanning the emission to get the real emission spectrum of the LED.
Regards,
Justin.
-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Julio Vazquez
Sent: Friday, 24 February 2012 4:14 AM
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Subject: Re: LEDs
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Hello all,
Applied Precision just told me that the InsightSSI illuminator is actually not LED based, so please ignore my previous posting, as far as LEDs are concerned.
Julio.
==
On Feb 23, 2012, at 2:06 AM, jacques wrote:
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> Thanks for your help and comments !
>
> Jacques