Protocols for fluorescent beads (fiducial markers)

Posted by Dan Metcalf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Protocols-for-fluorescent-beads-fiducial-markers-tp7365190.html

I've been using Invitrogen's 'fluospheres' but they are too bright for my application (STORM super-resolution microscopy). I'm thinking about making my own so that I can choose which dyes to use and can titrate down the amount of dye. Alternatively I could deliberately bleach the Invitrogen fluospheres I guess.

Can anyone point me in the direction of protocols for:

(1) Staining ~200 nm beads with dyes
(2) Bleaching a large aliquot of beads in a relatively uniform way

Thanks,

Dan Metcalf,
 
National Physical Laboratory
Teddington
UK