Posted by
Johannes Schindelin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-I-FOUND-the-little-SQUARES-fwd-tp7474216.html
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Hi list,
Sorry, was sending from the wrong address, so the list rejected it.
Therefore I am resending.
But this gives me the opportunity to state my point more clearly:
I do not take exception with displaying pixels as squares if that
helps analyze the data.
I do take exception with people pretending that pixels _are_
square. They are not.
I saw my share of bad analyses that were based on that false notion that
pixels have an exact square-shaped extent (or for that matter, voxels that
have an exact cuboid-shaped extent). For example, volume estimates of
reconstructed objects of interest were wildly off because of that (and
were reported without any indication of the expected precision), it was
not even funny.
Ciao,
Johannes
---------- Forwarded message ----------
Date: Tue, 17 Apr 2012 10:21:04 -0500 (CDT)
From: Johannes Schindelin <
[hidden email]>
To: "O'Malley, Donald" <
[hidden email]>
Cc:
[hidden email]
Subject: Re: [CONFOCALMICROSCOPY] I FOUND the little SQUARES
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Hi Donald,
On Tue, 17 Apr 2012, O'Malley, Donald wrote:
> I get the point that the sharp boundaries we see between adjacent pixels
> are physically impossible (in this case, for sure), but seeing the
> actual recorded data, presented as recorded, was useful at least to me.
> Of course, the colors in the images are a figment of our artistic
> imaginations!
It is okay to visualise your data in whatever form gives you the most
insight. For example, I am a total fan of lookup tables (e.g. the Glasbey
LUT to catch, uhm, "creative" use of Photoshop). We are never looking at
the original data again, after all, when we look at digital images. The
important point is -- as you did -- to keep in mind what those pixel
values actually stand for (and to make it easy for others to keep that in
mind, too).
Ciao,
Johannes