Posted by
JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/sample-prep-for-intra-occular-lenses-tp7482155p7482188.html
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This is an interesting puzzle. Those people who are working with curved
surfaces, such as retinas, make a series of radial cuts that allow them to
make flat(er) mounts. Of course, in this case, I would imagine that the
lenses are also of variable thickness, so there might be an additional
complication. You should be sure that the cellular surface is closest to
the cover slip so that the layer of cells is more uniform. Do you know on
which surface of the lens the cells will be found? Or are they on both?
Joel
On Thu, Apr 19, 2012 at 4:41 PM, Cromey, Douglas W - (dcromey) <
[hidden email]> wrote:
> I have a new set of users for our inverted confocal that will be bringing
> intra-occular lenses (of the kind used to fix cateracts). The lenses have
> a thin layer of cells on the surface, which is curved. Right now they are
> bringing them mounted in buffer as a whole mount with wax to seal the
> coverslip edges. They try to compress the lens by putting the slide under
> a book after it's mounted.
>
> It seems like there ought to be a better way and I'm hoping that someone
> out there has dealt with this before.
>
> Thanks!
> Doug
>
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Assistant Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
>
> office: AHSC 4212 email:
[hidden email]
> voice: 520-626-2824 fax: 520-626-2097
>
>
http://swehsc.pharmacy.arizona.edu/exppath/> Home of: "Microscopy and Imaging Resources on the WWW"
>
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--
Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
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