Posted by
Paul Herzmark on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cooling-time-for-CCD-cameras-tp7554139p7554473.html
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Esteban,
I am curious what kind of sample you expose for 30-60 seconds? Won't
background fluorescence kill you?
Paul Herzmark
Department of Molecular and Cell Biology
University of California, Berkeley
On Sat, May 12, 2012 at 2:28 PM, G. Esteban Fernandez <
[hidden email]> wrote:
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> Hi all,
>
> 1) I'm curious how much time, roughly, it takes for a Peltier-cooled
> CCD camera to reach the lowest temperature. Are we talking a few
> minutes or closer to an hour or more? I have several brands of cooled
> CCDs of different vintages and I'm just looking for a "rule of thumb",
> if possible.
>
> 2) I do plan to test my cameras myself: I was thinking of imaging a
> timelapse of a dim & stable specimen (requiring something like 30-60
> sec. exposure) after turning cooling on to see at what time noise
> reaches a minimum. Any suggestions for other ways to test this are
> welcome.
>
> Thanks,
> Esteban
>