Re: 3D reconstruction from serial sections

Posted by Peter Gabriel Pitrone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/3D-reconstruction-from-serial-sections-tp7557629p7559795.html

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Howdy Manuel,

This tool is already incorporated into TrakEM2 (http://fiji.sc/wiki/index.php/TrakEM2) using an elastic alignment (http://fiji.sc/wiki/index.php/Elastic_Alignment_and_Montage) Here are some practical examples:

http://fly.mpi-cbg.de/~saalfeld/video/elastic2.mov

http://fly.mpi-cbg.de/~saalfeld/video/elastic2-reslice.mov

I hope that this helps,

Pete

Peter Gabriel Pitrone TechRMS
Microscopy & Imaging Specialist
in Professor Pavel Tomancak's group
at Max Planck Institute for Molecular
Cell Biology and Genetics, Dresden
Pfotenhauerstrasse 108
01307, Dresden
Germany

[phone]
[hidden email]
https://wiki.mpi-cbg.de/wiki/openspim




On May 14, 2012, at 5:13 AM, Manuel Pele wrote:

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>
> Hi everyone,
>
> I'm looking for a way to reconstruct a full 3D image of my samples that I
> need to image using serial sections. Any tips about which software I can
> use ? Does it work well?
>
> Cheers
>
> Manuel
>
> --
> Manuel PELE, PhD
>
> Australian Regenerative Medicine Institute,
> Building 75, Level 1
> Monash University, Clayton Campus
> Wellington Road
> Clayton, VIC 3800
> AUSTRALIA
>
> Ph: [phone]
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