Deep tissue imaging with spinning disc - comparison with other confocal techniques

Posted by Jan Pala on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear listservers,

looking for some application techniques with deep tissue imaging I found
this article:
http://neuronet.jp/pdf/O_101.pdf
Journal of Integrative Neuroscience, Vol. 10, No. 1 (2011) 121–129
DOI: 10.1142/S0219635211002658
NIPKOW CONFOCAL IMAGING FROM DEEP BRAIN TISSUES
YUJI TAKAHARA, NORIO MATSUKI and YUJI IKEGAYA

where the authors describe advantages of spinning disc in comparison with
standard confocal laser scanning microscope and two-photon microscope.

What do  you think about presented results and experimental conditions? For
me, it is quite strange, see part 3.1:
1. the laser power was fixed at 5 mW in all microscope systems - for CLSM
the power is too high, for multiphoton too low. There is no description
about the way the power was measured and set
2. Real Z-resolution for all the systems
3. Results: CLSM penetration depth 80 microns, spinning disc penetration
depth 150 microns and multiphoton penetration depth 200 microns - it is
quite unbelieveable to see almost double penetration depth in between
spinning disc and CLSM.

Thank you in advance.

Best regards,
Jan Pala