http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610p7561706.html
you are asking our opinion on an article. But the way you are asking is already guiding into a specific direction. I personally do not like what you are trying to do (for me it goes into the direction of paper "bashing"). If you have questions/doubts regarding this technique or the results you should address it to the authors of the paper. Maybe it is just a misunderstanding (I cannot download the pdf properly so it is hard to judge) which could be ruled out by looking at the original data.
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]] On Behalf Of Jan Pala
> Sent: mercredi 16 mai 2012 12:50
> To:
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> Subject: Deep tissue imaging with spinning disc - comparison with other
> confocal techniques
>
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> Dear listservers,
>
> looking for some application techniques with deep tissue imaging I found this
> article:
>
http://neuronet.jp/pdf/O_101.pdf> Journal of Integrative Neuroscience, Vol. 10, No. 1 (2011) 121-129
> DOI: 10.1142/S0219635211002658
> NIPKOW CONFOCAL IMAGING FROM DEEP BRAIN TISSUES YUJI TAKAHARA,
> NORIO MATSUKI and YUJI IKEGAYA
>
> where the authors describe advantages of spinning disc in comparison with
> standard confocal laser scanning microscope and two-photon microscope.
>
> What do you think about presented results and experimental conditions?
> For me, it is quite strange, see part 3.1:
> 1. the laser power was fixed at 5 mW in all microscope systems - for CLSM the
> power is too high, for multiphoton too low. There is no description about the
> way the power was measured and set 2. Real Z-resolution for all the systems
> 3. Results: CLSM penetration depth 80 microns, spinning disc penetration
> depth 150 microns and multiphoton penetration depth 200 microns - it is
> quite unbelieveable to see almost double penetration depth in between
> spinning disc and CLSM.
>
> Thank you in advance.
>
> Best regards,
> Jan Pala