Re: commercial TIRF system

Posted by Csúcs Gábor on
URL: http://confocal-microscopy-list.275.s1.nabble.com/commercial-TIRF-system-tp7564151p7564206.html

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Dear Jean-Pierre,

This is a difficult question as currently there are at least 10 turn-key
system providers and the actual choice depends very much on your budget,
preference in terms of software/hardware functionality and of course the
available service at your location. I can tell you the following:

1) 2 years ago we looked quite carefully at the field and and have chosen
the Leica solution, because probably this is one of the systems which is
the easiest to operate in a core facility and it has quite a number of
nice innovations that are (or were at that time) missing from other
systems.

2) Today I'd certainly take a look at the Till-photonics system and the
Applied Precision one (although I don't know whether they sell their
beam-rotating system outside of the OMX platform), because these are the
two commercial providers (I'm aware of) who really rotate the laser beam
at the back focal plane of the objective (the Leica systems can also
rotate it, but at a much lover speed) and hence get a very homogenous
illumination.

3) Of course if your want to do "only" single particle tracking, then the
field homogeneity is much less important and probably any commercial
system where you have a back-illuminated EM-CCD as detector will give you
the sensitivity you need.

Greetings    Gabor

Light Microscopy and Screening Centre
ETH Zurich, HPM G41
Schafmattstrasse 18
CH-8093, Zurich
Switzerland

Phone: [phone]
Mobile: [phone]




On 5/17/12 3:38 AM, "Jean-Pierre CLAMME"
<[hidden email]> wrote:

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>
>Hi,
>
>I would like to get inputs about commercial turn Key, TIRF systems for
>single molecule particle tracking. I'm looking for a system that is
>relatively easy to operate and could be put in a core facility.
>
>Please let me know what you think about some you worked with and liked or
>disliked.
>
>Thank you
>
>JP
>
>
>- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
>Jean-Pierre CLAMME, PhD
>Chief Scientist
>Nitto Denko Technical
>501 Via Del Monte
>Oceanside, CA 92058
>E-mail: [hidden email]
>Phone: [phone]