Posted by
Sebastian Rhode on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610p7564998.html
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Hi Jan,
since our company manufactures both kind of systems, spinning disc confocal
and 2-Photon systems, just my opinion on this paper.
What I have seen so far during our tests, shows a clear advantage of 2PM if
depth penetration is your primary(!) goal. If it comes to speed, spinning
disc confocal (SDC) systems have a clear advantage. And in terms of cell
viability, 2PM and SDC are better than a conventional LSM microscope.
What puzzles me a bit, is the comparison between the penetration depth of
the SDC and LSM. There are several points (some of them already mentioned by
George here), which makes it difficult for me to really compare the results.
But nevertheless the findings are quite interesting, even if there are open
questions to answer. But I would be really interested in the explanation
about the observed results.
Dr. Sebastian Rhode
Project Manager Research & Development
TILL Photonics GmbH
an FEI Company