Posted by
Jan Pala on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610p7565201.html
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Dear Sebastian,
I agree with George and you. I still do not understand the excitation
comparison - based on your experience, is there somehow comparable the
excitation laser power in between different confocal principles (5 mW) as
presented in the paper?
For sure, I am also very interested in some deep explanation and analysis
of observed and presented results.
Thank you.
Best regards,
Jan
On Fri, May 18, 2012 at 9:10 AM, Sebastian Rhode <
[hidden email]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi Jan,
>
> since our company manufactures both kind of systems, spinning disc confocal
> and 2-Photon systems, just my opinion on this paper.
>
> What I have seen so far during our tests, shows a clear advantage of 2PM if
> depth penetration is your primary(!) goal. If it comes to speed, spinning
> disc confocal (SDC) systems have a clear advantage. And in terms of cell
> viability, 2PM and SDC are better than a conventional LSM microscope.
>
> What puzzles me a bit, is the comparison between the penetration depth of
> the SDC and LSM. There are several points (some of them already mentioned
> by
> George here), which makes it difficult for me to really compare the
> results.
>
> But nevertheless the findings are quite interesting, even if there are open
> questions to answer. But I would be really interested in the explanation
> about the observed results.
>
> Dr. Sebastian Rhode
> Project Manager Research & Development
>
> TILL Photonics GmbH
> an FEI Company
>
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