Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610p7565208.html
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MPEF can also be fast with resonant scanner (ex. Leica) or TriMScope
(LaVision Biotec, which uses array of spots and camera ... more spots,
less depth).
For example of fast resonant scanner with confocal see:
High-resolution, noninvasive longitudinal live imaging of immune
responses. </pubmed/21768391> Abdulreda MH, Faleo G, Molano RD,
Lopez-Cabezas M, Molina J, Tan Y, Echeverria OA, Zahr-Akrawi E,
Rodriguez-Diaz R, Edlund PK, Leibiger I, Bayer AL, Perez V, Ricordi C,
Caicedo A, Pileggi A, Berggren PO. Proc Natl Acad Sci U S A. 2011 Aug
2;108(31):12863-8. Epub 2011 Jul 18. PMID: 21768391.
This paper just used confocal lasers. If someone will send us money for
four HyD detectors NDD module, I'll get my colleagues to use our
Chameleon Ultra II more often.
On 5/18/2012 3:10 AM, Sebastian Rhode wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi Jan,
>
> since our company manufactures both kind of systems, spinning disc confocal
> and 2-Photon systems, just my opinion on this paper.
>
> What I have seen so far during our tests, shows a clear advantage of 2PM if
> depth penetration is your primary(!) goal. If it comes to speed, spinning
> disc confocal (SDC) systems have a clear advantage. And in terms of cell
> viability, 2PM and SDC are better than a conventional LSM microscope.
>
> What puzzles me a bit, is the comparison between the penetration depth of
> the SDC and LSM. There are several points (some of them already mentioned by
> George here), which makes it difficult for me to really compare the results.
>
> But nevertheless the findings are quite interesting, even if there are open
> questions to answer. But I would be really interested in the explanation
> about the observed results.
>
> Dr. Sebastian Rhode
> Project Manager Research& Development
>
> TILL Photonics GmbH
> an FEI Company
>
>