Re: Deep tissue imaging with spinning disc - comparison with other confocal techniques

Posted by Sebastian Rhode on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deep-tissue-imaging-with-spinning-disc-comparison-with-other-confocal-techniques-tp7561610p7565509.html

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Hi Mark,

shame on me, that did not really read the paper. So when using a 20X NA0.95
objective, the required pinhole should have been ~13 micron in diameter
instead of the used 50 microns. We use 30 micron right now a I have not
looked really deep into our brain slices using our Andromeda SDC system.

Cheers,

Sebi