Fluorescent stain for leukocytes

Posted by B. Prabhakar Pandian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Bleaching-of-background-tp7578651p7578660.html

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Hello,
            Can someone suggest a stable fluorescent stain for live cell
imaging of leukocytes upto 4 hours.  We are using Rhodamine but the
signal goes away rapidly.
We keep the lamp off during the non-acquisition time, hence
photo-bleaching is not the main issue.

Thanks,

-Prabhakar